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Friday, March 18, 2011

HOW IS MONOCLONAL ANTIBODY PRODUCED EXACTLY

Friday, March 18, 2011



Process by which large quantities of antibodies (targeted against a particular antigen X) can be produced.

A mouse is immunized by injection of an antigen X to stimulate the production of antibodies targeted against X. The antibody forming cells are isolated from the mouse's spleen.

Monoclonal antibodies are produced by fusing single antibody-forming cells to tumor cells grown in culture. The resulting cell is called a hybridoma.

Each hybridoma produces relatively large quantities of identical antibody molecules. By allowing the hybridoma to multiply in culture, it is possible to produce a population of cells, each of which produces identical antibody molecules. These antibodies are called "monoclonal antibodies" because they are produced by the identical offspring of a single, cloned antibody producing cell.

Once a monoclonal antibody is made, it can be used as a specific probe to track down and purify the specific protein that induced its formation

6 comments:

antibody said...

Monoclonal antibodies have changed the rules of the game in our favor. It is now generally accepted that the majority of the cell killing gets done by the body's own immune system. Each hybridoma produces relatively large quantities of identical antibody molecules. Thanks...

dna equipment said...

thanks do you know that monoclonal antibody industry has been doing good result by now ?

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Innate Immunity said...

Hello,

Thank you for the good writeup. Monoclonal antibodies are an essential part of a molecular biologist's toolkit. These Antibodies are proteins produced by the B lymphocytes of the immune system in response to foreign proteins...

Metabolism Assays said...

Hi, thank you for sharing such detailed information! It's really useful!

Antibiotics said...

the production process of antibody is a little complicated. you have explained it in such a simple way.

 

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