He..he...
Today I would like to give you 'teaching' how to isolate DNA in your
own home by yourself with your any kitchen tools in your home. Yesterday I read some resource that it is worth to have a reading. OK lets make your first experiment how to isolate DNA by yourself easily...
1. First, you need to find something that contains DNA. Since DNA is the blueprint for life, everything living contains DNA. For this experiment, we like to use green split peas. But there are lots of other DNA sources too, such as: Spinach, chicken liver, meal, chicken lungs, or what ever....
2. You can not use your stone, gold, silver, mug, to do it as it is not live things...
3. 3. Put in a blender : 1/2 cup of split peas (100ml) , 1/8 teaspoon table salt (less than 1ml), 1 cup cold water (200ml). Blend on high for 15 seconds. The blender separates the pea cells from each other, so you now have a really thin pea-cell soup.
4. Pour your thin pea-cell soup through a strainer into another container (like a measuring cup).
Add 2 tablespoons liquid detergent (about 30ml) and swirl to mix. Let the mixture sit for 5-10 minutes. Pour the mixture into test tubes or other small glass containers, each about 1/3 full.
5. Add a pinch of enzymes to each test tube and stir gently. Be careful! If you stir too hard, you'll break up the DNA, making it harder to see. Use meat tenderizer for enzymes. If you can't find tenderizer, try using pineapple juice or contact lens cleaning solution...
* In this experiment, meat tenderizer acts as an enzyme to cut proteins just like a pair of scissors. The DNA in the nucleus of the cell is molded, folded, and protected by proteins. The meat tenderizer cuts the proteins away from the DNA.
6.Tilt your test tube and slowly pour rubbing alcohol (70-95% isopropyl or ethyl alcohol) into the tube down the side so that it forms a layer on top of the pea mixture. Pour until you have about the same amount of alcohol in the tube as pea mixture.
DNA will rise into the alcohol layer from the pea layer. You can use a wooden stick or other hook to draw the DNA into the alcohol.
7. What is that stringy stuff ??. Alcohol is less dense than water, so it floats on top. Since two separate layers are formed, all of the grease and the protein that we broke up in the first two steps and the DNA have to decide: "Hmmm...which layer should I go to?"
This is sort of like looking around the room for the most comfortable seat. Some will choose the couch, others might choose the rocking chair.
In this case, the protein and grease parts find the bottom, watery layer the most comfortable place, while the DNA prefers the top, alcohol layer.
DNA is a long, stringy molecule that likes to clump together.
8. Now that you've successfully extracted DNA from one source, you're ready to experiment further. Try these ideas or some of your own:
Experiment with other DNA sources. Which source gives you the most DNA? How can you compare them?
Experiment with different soaps and detergents. Do powdered soaps work as well as liquid detergents? How about shampoo or body scrub?
Experiment with leaving out or changing steps. We've told you that you need each step, but is this true? Find out for yourself. Try leaving out a step or changing how much of each ingredient you use.
Do only living organisms contain DNA? Try extracting DNA from things that you think might not have DNA.
Hooray...you get the DNA and you already become scientist...Congratulation.....!!!
Today I would like to give you 'teaching' how to isolate DNA in your
own home by yourself with your any kitchen tools in your home. Yesterday I read some resource that it is worth to have a reading. OK lets make your first experiment how to isolate DNA by yourself easily...
1. First, you need to find something that contains DNA. Since DNA is the blueprint for life, everything living contains DNA. For this experiment, we like to use green split peas. But there are lots of other DNA sources too, such as: Spinach, chicken liver, meal, chicken lungs, or what ever....
2. You can not use your stone, gold, silver, mug, to do it as it is not live things...
3. 3. Put in a blender : 1/2 cup of split peas (100ml) , 1/8 teaspoon table salt (less than 1ml), 1 cup cold water (200ml). Blend on high for 15 seconds. The blender separates the pea cells from each other, so you now have a really thin pea-cell soup.
4. Pour your thin pea-cell soup through a strainer into another container (like a measuring cup).
Add 2 tablespoons liquid detergent (about 30ml) and swirl to mix. Let the mixture sit for 5-10 minutes. Pour the mixture into test tubes or other small glass containers, each about 1/3 full.
5. Add a pinch of enzymes to each test tube and stir gently. Be careful! If you stir too hard, you'll break up the DNA, making it harder to see. Use meat tenderizer for enzymes. If you can't find tenderizer, try using pineapple juice or contact lens cleaning solution...
* In this experiment, meat tenderizer acts as an enzyme to cut proteins just like a pair of scissors. The DNA in the nucleus of the cell is molded, folded, and protected by proteins. The meat tenderizer cuts the proteins away from the DNA.
6.Tilt your test tube and slowly pour rubbing alcohol (70-95% isopropyl or ethyl alcohol) into the tube down the side so that it forms a layer on top of the pea mixture. Pour until you have about the same amount of alcohol in the tube as pea mixture.
DNA will rise into the alcohol layer from the pea layer. You can use a wooden stick or other hook to draw the DNA into the alcohol.
7. What is that stringy stuff ??. Alcohol is less dense than water, so it floats on top. Since two separate layers are formed, all of the grease and the protein that we broke up in the first two steps and the DNA have to decide: "Hmmm...which layer should I go to?"
This is sort of like looking around the room for the most comfortable seat. Some will choose the couch, others might choose the rocking chair.
In this case, the protein and grease parts find the bottom, watery layer the most comfortable place, while the DNA prefers the top, alcohol layer.
DNA is a long, stringy molecule that likes to clump together.
8. Now that you've successfully extracted DNA from one source, you're ready to experiment further. Try these ideas or some of your own:
Experiment with other DNA sources. Which source gives you the most DNA? How can you compare them?
Experiment with different soaps and detergents. Do powdered soaps work as well as liquid detergents? How about shampoo or body scrub?
Experiment with leaving out or changing steps. We've told you that you need each step, but is this true? Find out for yourself. Try leaving out a step or changing how much of each ingredient you use.
Do only living organisms contain DNA? Try extracting DNA from things that you think might not have DNA.
Hooray...you get the DNA and you already become scientist...Congratulation.....!!!
